Characterization of Nanobubbles Based on the Type of Gas Used
- Research Duration : 2024–2025
- Funding : Reverse Aging and Homeostasis (RAHO) Club
Background
Nanobubbles (NBs) are gas bubbles with a diameter of less than 200 nm, exhibiting unique characteristics compared to micro - or macro bubbles. These include high stability in liquids, the potential to penetrate biological tissues, and efficient gas transfer capacity. In various studies, NBs have been applied in the biomedical field, as well as in agriculture, environmental applications, and experimental therapies such as hydrogen and oxygen therapy for inflammatory and metabolic conditions. One of the main challenges in developing NB technology is understanding the relationship between the type of gas used, NB concentration, size distribution, and its stability in specific media. Comprehensive characterization of these parameters is essential to optimize the performance of NBs in various applications.
In colloidal systems based on pure water (H₂O) and physiological saline (NS), the structure and dynamics of NBs can be influenced by the physicochemical properties of the media, such as osmotic pressure, viscosity, and ionic content. Differences in media composition may affect average size, polydispersity distribution, and the rate of NB degradation or coalescence over time. Additionally, the type of gas used—such as H₂, O₂, or a combination of both—has the potential to modify interactions between NBs and between NBs and their surrounding fluid environment, which directly impacts the short- and long-term stability of the system.
This study aims to characterize NBs based on the type of gas used, measure the concentration and size distribution of NBs, and evaluate their stability in two colloidal media: H₂O and NS. The relationships among these factors are expected to provide deeper insight into the behavior of NBs in simplified biological systems and serve as a foundation for the controlled and efficient development of nanobubble-based therapies or technologies.
Research Highlights
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Nanobubbles (NBs) have unique characteristics such as high stability in liquids and the ability to penetrate biological tissues, making them promising for medical applications, including hydrogen and oxygen therapies.
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The stability and performance of NBs are influenced by several factors, including the type of gas used (H₂, O₂, or a combination), concentration, size distribution, and the suspension media.
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Colloidal media such as pure water (H₂O) and physiological saline (NS) possess different physicochemical properties, such as osmotic pressure and ionic content, which can affect NB structure, size, and degradation rate.
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This research focuses on comprehensive characterization of NBs as an initial step toward developing more controlled and efficient NB-based applications in the health sector.
Goals
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To characterize nanobubbles (NBs) based on the type of gas used, including hydrogen (H₂), oxygen (O₂), and their combination.
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To measure the concentration and size distribution of NBs for each gas type in two colloidal media: pure water (H₂O) and physiological saline (NS).
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To evaluate the stability of nanobubbles in both media over storage time.
Expected Results
This study is expected to provide a comprehensive understanding of nanobubble characteristics based on gas type, concentration, size distribution, and stability in H₂O and physiological saline media. It is anticipated that the research will reveal how different gas types and media compositions influence the physicochemical behavior of nanobubbles, such as stability and coalescence tendencies. These findings are expected to serve as a strong scientific foundation for guiding the development of nanobubble applications in the healthcare field, particularly in the formulation of more effective and controlled gas-based therapies.
Article Link: https://f1000research.com/articles/14-406